7B0U の概要
| エントリーDOI | 10.2210/pdb7b0u/pdb |
| EMDBエントリー | 11971 |
| 分子名称 | RsbR protein, RsbS protein, ... (5 entities in total) |
| 機能のキーワード | stressosome, stress sensing, general stress response, phosphorylation cascade, signaling protein |
| 由来する生物種 | Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) 詳細 |
| タンパク質・核酸の鎖数 | 60 |
| 化学式量合計 | 1522557.02 |
| 構造登録者 | |
| 主引用文献 | Miksys, A.,Fu, L.,Madej, M.G.,Guerreiro, D.N.,Kaltwasser, S.,Conway, M.,Ejder, S.,Bruckmann, A.,Marles-Wright, J.,Lewis, R.J.,O'Byrne, C.,Pane-Farre, J.,Ziegler, C. Molecular insights into intra-complex signal transmission during stressosome activation. Commun Biol, 5:621-621, 2022 Cited by PubMed Abstract: The stressosome is a pseudo-icosahedral megadalton bacterial stress-sensing protein complex consisting of several copies of two STAS-domain proteins, RsbR and RsbS, and the kinase RsbT. Upon perception of environmental stress multiple copies of RsbT are released from the surface of the stressosome. Free RsbT activates downstream proteins to elicit a global cellular response, such as the activation of the general stress response in Gram-positive bacteria. The molecular events triggering RsbT release from the stressosome surface remain poorly understood. Here we present the map of Listeria innocua RsbR1/RsbS complex at resolutions of 3.45 Å for the STAS domain core in icosahedral symmetry and of 3.87 Å for the STAS domain and N-terminal sensors in D2 symmetry, respectively. The structure reveals a conformational change in the STAS domain linked to phosphorylation in RsbR. Docking studies indicate that allosteric RsbT binding to the conformationally flexible N-terminal sensor domain of RsbR affects the affinity of RsbS towards RsbT. Our results bring to focus the molecular events within the stressosome complex and further our understanding of this ubiquitous signaling hub. PubMed: 35760945DOI: 10.1038/s42003-022-03549-9 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.86 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
