7AXZ

Ku70/80 complex apo form

7AXZ の概要

• エントリーDOI: 10.2210/pdb7axz/pdb
• EMDBエントリー: 11933
• 分子名称: X-ray repair cross-complementing protein 6, X-ray repair cross-complementing protein 5 (2 entities in total)
• 機能のキーワード: nhej, ku70/80, xrcc5, xrcc6, dna damage, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 152757.48

構造登録者

Hnizda, A.,Tesina, P.,Novak, P.,Blundell, T.L. (登録日: 2020-11-10, 公開日: 2021-02-10, 最終更新日: 2024-05-01)

主引用文献

Hnizda, A.,Tesina, P.,Nguyen, T.B.,Kukacka, Z.,Kater, L.,Chaplin, A.K.,Beckmann, R.,Ascher, D.B.,Novak, P.,Blundell, T.L.
SAP domain forms a flexible part of DNA aperture in Ku70/80.
Febs J., 288:4382-4393, 2021

PubMed Abstract: Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C-terminal domain, known as the SAP domain. Using single-particle cryo-electron microscopy, mass spectrometric analysis of intermolecular cross-linking and molecular modelling simulations, we captured variable positions of the SAP domain depending on DNA binding. The first position was localized at the DNA aperture in the Ku70/80 apo form but was not observed in the DNA-bound state. The second position, which was observed in both apo and DNA-bound states, was found below the DNA aperture, close to the helical arm of Ku70. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. DATABASES: EM maps have been deposited in EMDB (EMD-11933). Coordinates have been deposited in Protein Data Bank (PDB 7AXZ). Other data are available from corresponding authors upon a request.

PubMed: 33511782
DOI: 10.1111/febs.15732

実験手法

ELECTRON MICROSCOPY (3.2 Å)