7AX0

Crystal structure of the computationally designed Scone-E protein co-crystallized with STA form a

7AX0 の概要

• エントリーDOI: 10.2210/pdb7ax0/pdb
• 分子名称: SconeE, Keggin (STA), PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: beta-propeller, computational design, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43180.98

構造登録者

Mylemans, B.,Vandebroek, L.,Parac-Vogt, T.N.,Voet, A.R.D. (登録日: 2020-11-09, 公開日: 2021-06-30, 最終更新日: 2024-01-31)

主引用文献

Mylemans, B.,Killian, T.,Vandebroek, L.,Van Meervelt, L.,Tame, J.R.H.,Parac-Vogt, T.N.,Voet, A.R.D.
Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.
Acta Crystallogr D Struct Biol, 77:933-942, 2021

PubMed Abstract: Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric β-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives.

PubMed: 34196619
DOI: 10.1107/S2059798321005787

実験手法

X-RAY DIFFRACTION (2.2 Å)