7AW9

CCAAT-binding complex and HapX bound to Aspergillus fumigatus cccA DNA

7AW9 の概要

• エントリーDOI: 10.2210/pdb7aw9/pdb
• 関連するPDBエントリー: 6Y36 6Y37 7AW7
• 分子名称: HapB, Transcription factor HapC (Eurofung), CBFD_NFYB_HMF domain-containing protein, ... (7 entities in total)
• 機能のキーワード: transcription factor, histone fold, basic leucine zipper, heteropentamer, protein-dna complex, transcription
• 由来する生物種: Aspergillus nidulans FGSC A4; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 77743.99

構造登録者

Huber, E.M.,Groll, M. (登録日: 2020-11-06, 公開日: 2022-04-13, 最終更新日: 2024-01-31)

主引用文献

Huber, E.M.,Hortschansky, P.,Scheven, M.T.,Misslinger, M.,Haas, H.,Brakhage, A.A.,Groll, M.
Structural insights into cooperative DNA recognition by the CCAAT-binding complex and its bZIP transcription factor HapX.
Structure, 30:934-, 2022

PubMed Abstract: The heterotrimeric CCAAT-binding complex (CBC) is a fundamental eukaryotic transcription factor recognizing the CCAAT box. In certain fungi, like Aspergilli, the CBC cooperates with the basic leucine zipper HapX to control iron metabolism. HapX functionally depends on the CBC, and the stable interaction of both requires DNA. To study this cooperative effect, X-ray structures of the CBC-HapX-DNA complex were determined. Downstream of the CCAAT box, occupied by the CBC, a HapX dimer binds to the major groove. The leash-like N terminus of the distal HapX subunit contacts the CBC, and via a flexible polyproline type II helix mediates minor groove interactions that stimulate sequence promiscuity. In vitro and in vivo mutagenesis suggest that the structural and functional plasticity of HapX results from local asymmetry and its ability to target major and minor grooves simultaneously. The latter feature may also apply to related transcription factors such as yeast Hap4 and distinct Yap family members.

PubMed: 35472306
DOI: 10.1016/j.str.2022.04.001

実験手法

X-RAY DIFFRACTION (3.5 Å)