7ATZ

G-quadruplex with V-shaped loop from the first repeat of KCNN4 minisatellite

7ATZ の概要

• エントリーDOI: 10.2210/pdb7atz/pdb
• NMR情報: BMRB: 34571
• 分子名称: DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3') (1 entity in total)
• 機能のキーワード: g-quadruplex, v-loop, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7298.68

構造登録者

Vianney, Y.M.,Weisz, K. (登録日: 2020-11-02, 公開日: 2021-04-21, 最終更新日: 2024-05-15)

主引用文献

Vianney, Y.M.,Weisz, K.
First Tandem Repeat of a Potassium Channel KCNN4 Minisatellite Folds into a V-Loop G-Quadruplex Structure.
Biochemistry, 60:1337-1346, 2021

PubMed Abstract: The gene encoding a potassium channel protein whose expression has been correlated with tumor progression was found to comprise a guanine-rich minisatellite region with the ability to form a putative G-quadruplex (G4). Given the suggested regulatory role of G4s in gene expression, G-quadruplex formation for the polymorphic first repeat of the minisatellite was studied by nuclear magnetic resonance spectroscopy. A stable G-quadruplex of a truncated mutant sequence was shown to represent one of several coexisting species of the wild-type sequence. The high-resolution structure features a noncanonical G4 with a broken G-column and a V-shaped loop. The presence of a 3'-flanking thymidine interacting with the lateral loop preceding the V loop seems to be critical for the formation of this G4 topology. On the contrary, an additional 5'-flanking residue disfavored but still allowed folding into the V-loop structure. The latter may therefore serve as a putative therapeutic target in strategies for G4-based modulation of expression.

PubMed: 33844501
DOI: 10.1021/acs.biochem.1c00043

実験手法

SOLUTION NMR