7AS4

Recombinant human gTuRC

これはPDB形式変換不可エントリーです。

7AS4 の概要

• エントリーDOI: 10.2210/pdb7as4/pdb
• EMDBエントリー: 11888
• 分子名称: Tubulin gamma-1 chain, Gamma-tubulin complex component 3, Gamma-tubulin complex component 6, ... (9 entities in total)
• 機能のキーワード: microtubule organizing center, microtubule, gamma-tubulin ring complex, gamma-tubulin small complex, spindle organization, microtubule nucleation, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 33
• 化学式量合計: 2583168.09

構造登録者

Serna, M.,Fernandez-Leiro, R.,Llorca, O. (登録日: 2020-10-26, 公開日: 2021-01-20, 最終更新日: 2024-05-01)

主引用文献

Zimmermann, F.,Serna, M.,Ezquerra, A.,Fernandez-Leiro, R.,Llorca, O.,Luders, J.
Assembly of the asymmetric human gamma-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.
Sci Adv, 6:-, 2020

PubMed Abstract: The microtubule nucleator γ-tubulin ring complex (γTuRC) is essential for the function of microtubule organizing centers such as the centrosome. Since its discovery over two decades ago, γTuRC has evaded in vitro reconstitution and thus detailed structure-function studies. Here, we show that a complex of RuvB-like protein 1 (RUVBL1) and RUVBL2 "RUVBL" controls assembly and composition of γTuRC in human cells. Likewise, RUVBL assembles γTuRC from a minimal set of core subunits in a heterologous coexpression system. RUVBL interacts with γTuRC subcomplexes but is not part of fully assembled γTuRC. Purified, reconstituted γTuRC has nucleation activity and resembles native γTuRC as revealed by its cryo-electron microscopy (cryo-EM) structure at ~4.0-Å resolution. We further use cryo-EM to identify features that determine the intricate, higher-order γTuRC architecture. Our work finds RUVBL as an assembly factor that regulates γTuRC in cells and allows production of recombinant γTuRC for future in-depth mechanistic studies.

PubMed: 33355144
DOI: 10.1126/sciadv.abe0894

実験手法

ELECTRON MICROSCOPY (4.13 Å)