7AOC

Schizosaccharomyces pombe RNA polymerase I (monomer)

7AOC の概要

• エントリーDOI: 10.2210/pdb7aoc/pdb
• EMDBエントリー: 11840
• 分子名称: DNA-directed RNA polymerase I subunit rpa1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (13 entities in total)
• 機能のキーワード: transcription
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 493737.18

構造登録者

Heiss, F.,Daiss, J.,Becker, P.,Engel, C. (登録日: 2020-10-14, 公開日: 2021-02-24, 最終更新日: 2024-10-16)

主引用文献

Heiss, F.B.,Daiss, J.L.,Becker, P.,Engel, C.
Conserved strategies of RNA polymerase I hibernation and activation.
Nat Commun, 12:758-758, 2021

PubMed Abstract: RNA polymerase (Pol) I transcribes the ribosomal RNA precursor in all eukaryotes. The mechanisms 'activation by cleft contraction' and 'hibernation by dimerization' are unique to the regulation of this enzyme, but structure-function analysis is limited to baker's yeast. To understand whether regulation by such strategies is specific to this model organism or conserved among species, we solve three cryo-EM structures of Pol I from Schizosaccharomyces pombe in different functional states. Comparative analysis of structural models derived from high-resolution reconstructions shows that activation is accomplished by a conserved contraction of the active center cleft. In contrast to current beliefs, we find that dimerization of the S. pombe polymerase is also possible. This dimerization is achieved independent of the 'connector' domain but relies on two previously undescribed interfaces. Our analyses highlight the divergent nature of Pol I transcription systems from their counterparts and suggest conservation of regulatory mechanisms among organisms.

PubMed: 33536435
DOI: 10.1038/s41467-021-21031-8

実験手法

ELECTRON MICROSCOPY (3.84 Å)