7ANU

Crystal structure of Pyrococcus abyssi Pbp11

これはPDB形式変換不可エントリーです。

7ANU の概要

• エントリーDOI: 10.2210/pdb7anu/pdb
• 分子名称: Pbp11 protein, NICKEL (II) ION, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: ob-fold, proteasome adaptor, trna binding, protein binding
• 由来する生物種: Pyrococcus abyssi (strain GE5 / Orsay)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21472.40

構造登録者

Hogrel, G.,Girard, E.,Franzetti, B. (登録日: 2020-10-12, 公開日: 2021-11-03, 最終更新日: 2024-01-31)

主引用文献

Hogrel, G.,Marino-Puertas, L.,Laurent, S.,Ibrahim, Z.,Coves, J.,Girard, E.,Gabel, F.,Fenel, D.,Daugeron, M.C.,Clouet-d'Orval, B.,Basta, T.,Flament, D.,Franzetti, B.
Characterization of a small tRNA-binding protein that interacts with the archaeal proteasome complex.
Mol.Microbiol., 118:16-29, 2022

PubMed Abstract: The proteasome system allows the elimination of functional or structurally impaired proteins. This includes the degradation of nascent peptides. In Archaea, how the proteasome complex interacts with the translational machinery remains to be described. Here, we characterized a small orphan protein, Q9UZY3 (UniProt ID), conserved in Thermococcales. The protein was identified in native pull-down experiments using the proteasome regulatory complex (proteasome-activating nucleotidase [PAN]) as bait. X-ray crystallography and small-angle X-ray scattering experiments revealed that the protein is monomeric and adopts a β-barrel core structure with an oligonucleotide/oligosaccharide-binding (OB)-fold, typically found in translation elongation factors. Mobility shift experiment showed that Q9UZY3 displays transfer ribonucleic acid (tRNA)-binding properties. Pull-downs, co-immunoprecipitation and isothermal titration calorimetry (ITC) studies revealed that Q9UZY3 interacts in vitro with PAN. Native pull-downs and proteomic analysis using different versions of Q9UZY3 showed that the protein interacts with the assembled PAN-20S proteasome machinery in Pyrococcus abyssi (Pa) cellular extracts. The protein was therefore named Pbp11, for Proteasome-Binding Protein of 11 kDa. Interestingly, the interaction network of Pbp11 also includes ribosomal proteins, tRNA-processing enzymes and exosome subunits dependent on Pbp11's N-terminal domain that was found to be essential for tRNA binding. Together these data suggest that Pbp11 participates in an interface between the proteasome and the translational machinery.

PubMed: 35615908
DOI: 10.1111/mmi.14948

実験手法

X-RAY DIFFRACTION (1.64 Å)