7AL3

Native-like genome-containing particle of DWV in acidic pH

7AL3 の概要

• エントリーDOI: 10.2210/pdb7al3/pdb
• EMDBエントリー: 11815
• 分子名称: Genome polyprotein (3 entities in total)
• 機能のキーワード: dwv, acidic ph, genome-containing, virus
• 由来する生物種: Deformed wing virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 103737.76

構造登録者

Skubnik, K.,Plevka, P. (登録日: 2020-10-04, 公開日: 2021-02-10, 最終更新日: 2024-05-01)

主引用文献

Skubnik, K.,Sukenik, L.,Buchta, D.,Fuzik, T.,Prochazkova, M.,Moravcova, J.,Smerdova, L.,Pridal, A.,Vacha, R.,Plevka, P.
Capsid opening enables genome release of iflaviruses.
Sci Adv, 7:-, 2021

PubMed Abstract: The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.

PubMed: 33523856
DOI: 10.1126/sciadv.abd7130

実験手法

ELECTRON MICROSCOPY (4.8 Å)