7AHN

Cryo-EM structure of F-actin stabilized by cis-optoJASP-8

7AHN の概要

• エントリーDOI: 10.2210/pdb7ahn/pdb
• EMDBエントリー: 11787
• 分子名称: Actin, alpha skeletal muscle, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: cytoskeleton, jasplakinolide, azobenzene photoswitch, stabilized-actin filament, structural protein
• 由来する生物種: Oryctolagus cuniculus (Rabbit)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 218648.45

構造登録者

Pospich, S.,Raunser, S. (登録日: 2020-09-24, 公開日: 2021-01-27, 最終更新日: 2025-04-09)

主引用文献

Pospich, S.,Kullmer, F.,Nasufovic, V.,Funk, J.,Belyy, A.,Bieling, P.,Arndt, H.D.,Raunser, S.
Cryo-EM Resolves Molecular Recognition Of An Optojasp Photoswitch Bound To Actin Filaments In Both Switch States.
Angew.Chem.Int.Ed.Engl., 60:8678-8682, 2021

PubMed Abstract: Actin is essential for key processes in all eukaryotic cells. Cellpermeable optojasps provide spatiotemporal control of the actin cytoskeleton, confining toxicity and potentially rendering F-actin druggable by photopharmacology. Here, we report cryo electron microscopy (cryo-EM) structures of both isomeric states of one optojasp bound to actin filaments. The high-resolution structures reveal for the first time the pronounced effects of photoswitching a functionalized azobenzene. By characterizing the optojasp binding site and identifying conformational changes within F-actin that depend on the optojasp isomeric state, we refine determinants for the design of functional F-actin photoswitches.

PubMed: 33449370
DOI: 10.1002/anie.202013193

実験手法

ELECTRON MICROSCOPY (2.9 Å)