7AGG

HAd7 knob in complex with 2 EC2-EC3 modules of DSG-2

7AGG の概要

• エントリーDOI: 10.2210/pdb7agg/pdb
• EMDBエントリー: 11778 11779
• 分子名称: Fiber, Desmoglein-2 (2 entities in total)
• 機能のキーワード: adenovirus, cell receptor, complex, viral protein
• 由来する生物種: Human adenovirus B serotype 7 (HAdV-7); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 124720.20

構造登録者

Effantin, G.,Vassal-Stermann, E.,Fender, P. (登録日: 2020-09-22, 公開日: 2020-10-14, 最終更新日: 2025-07-09)

主引用文献

Hograindleur, M.A.,Effantin, G.,Fenel, D.,Mas, C.,Lieber, A.,Schoehn, G.,Fender, P.,Vassal-Stermann, E.
Binding Mechanism Elucidation of the Acute Respiratory Disease Causing Agent Adenovirus of Serotype 7 to Desmoglein-2.
Viruses, 12:-, 2020

PubMed Abstract: The study of viruses causing acute respiratory distress syndromes (ARDS) is more essential than ever at a time when a virus can create a global pandemic in a matter of weeks. Among human adenoviruses, adenovirus of serotype 7 (HAdV7) is one of the most virulent serotypes. This virus regularly re-emerges in Asia and has just been the cause of several deaths in the United States. A critical step of the virus life cycle is the attachment of the knob domain of the fiber (HAd7K) to the cellular receptor desmoglein-2 (DSG2). Complexes between the fiber knob and two extracellular domains of DSG2 have been produced. Their characterization by biochemical and biophysical methods show that these two domains are sufficient for the interaction and that the trimeric HAd7K could accommodate up to three DSG2 receptor molecules. The cryo-electron microscopy (cryo-EM) structure of these complexes at 3.1 Å resolution confirmed the biochemical data, and allowed the identification of the critical amino acid residues for this interaction, which shows similarities with other DSG2 interacting adenoviruses, despite a low homology in the primary sequences.

PubMed: 32992715
DOI: 10.3390/v12101075

実験手法

ELECTRON MICROSCOPY (3.3 Å)