7AFL

Bacterial 30S ribosomal subunit assembly complex state D (multibody refinement for body domain of 30S ribosome)

7AFL の概要

• エントリーDOI: 10.2210/pdb7afl/pdb
• EMDBエントリー: 11769
• 分子名称: 16SrRNA, 30S ribosomal protein S17, 30S ribosomal protein S18, ... (15 entities in total)
• 機能のキーワード: cryo-em, 30s biogenesis, ribosome assembly, rbfa, rsga, yjeq, rimp, ksga, rsma, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 679035.23

構造登録者

Schedlbauer, A.,Iturrioz, I.,Ochoa-Lizarralde, B.,Diercks, T.,Kaminishi, T.,Capuni, R.,Astigarraga, E.,Gil-Carton, D.,Fucini, P.,Connell, S. (登録日: 2020-09-19, 公開日: 2021-07-07, 最終更新日: 2024-04-24)

主引用文献

Schedlbauer, A.,Iturrioz, I.,Ochoa-Lizarralde, B.,Diercks, T.,Lopez-Alonso, J.P.,Lavin, J.L.,Kaminishi, T.,Capuni, R.,Dhimole, N.,de Astigarraga, E.,Gil-Carton, D.,Fucini, P.,Connell, S.R.
A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
Sci Adv, 7:-, 2021

PubMed Abstract: While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30 ribosomal subunit and obtained eight snapshots representing late steps in the folding of the decoding center. Analysis of these structures identifies a conserved secondary structure switch in the 16 ribosomal RNA central to decoding site maturation and suggests both a sequential order of action and molecular mechanisms for the assembly factors in coordinating and controlling this switch. Structural and mechanistic parallels between bacterial and eukaryotic systems indicate common folding features inherent to all ribosomes.

PubMed: 34088665
DOI: 10.1126/sciadv.abf7547

実験手法

ELECTRON MICROSCOPY (4.2 Å)