7AE0

Cryo-EM structure of an extracellular contractile injection system in marine bacterium Algoriphagus machipongonensis, the sheath-tube module in extended state.

これはPDB形式変換不可エントリーです。

7AE0 の概要

• エントリーDOI: 10.2210/pdb7ae0/pdb
• EMDBエントリー: 11734 11735
• 分子名称: Putative phage tail sheath protein FI, Phage tail protein (2 entities in total)
• 機能のキーワード: extracellular contractile injection system, structural protein
• 由来する生物種: Algoriphagus machipongonensis; 詳細
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1668500.95

構造登録者

Xu, J.,Ericson, C.,Feldmueller, M.,Lien, Y.W.,Pilhofer, M. (登録日: 2020-09-17, 公開日: 2022-02-16, 最終更新日: 2024-07-10)

主引用文献

Xu, J.,Ericson, C.F.,Lien, Y.W.,Rutaganira, F.U.N.,Eisenstein, F.,Feldmuller, M.,King, N.,Pilhofer, M.
Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.
Nat Microbiol, 7:397-410, 2022

PubMed Abstract: Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). Bioinformatic studies uncovered a phylogenetic group of hundreds of putative CIS gene clusters that are highly diverse and widespread; however, only four systems have been characterized. Here we studied a putative CIS gene cluster in the marine bacterium Algoriphagus machipongonensis. Using an integrative approach, we show that the system is compatible with an eCIS mode of action. Our cryo-electron microscopy structure revealed several features that differ from those seen in other CISs: a 'cap adaptor' located at the distal end, a 'plug' exposed to the tube lumen, and a 'cage' formed by massive extensions of the baseplate. These elements are conserved in other CISs, and our genetic tools identified that they are required for assembly, cargo loading and function. Furthermore, our atomic model highlights specific evolutionary hotspots and will serve as a framework for understanding and re-engineering CISs.

PubMed: 35165385
DOI: 10.1038/s41564-022-01059-2

実験手法

ELECTRON MICROSCOPY (2.4 Å)