7ADM

Structure of the mycoplasma MIB protein

7ADM の概要

• エントリーDOI: 10.2210/pdb7adm/pdb
• EMDBエントリー: 11729 11731
• 分子名称: Putative immunoglobulin-blocking virulence protein (1 entity in total)
• 機能のキーワード: antibody binding protein, protease, protein complex., membrane protein
• 由来する生物種: Mycoplasma mycoides subsp. capri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 84962.72

構造登録者

Nottelet, P.,Bataille, L.,Gourgues, G.,Anger, R.,Lartigue, C.,Sirand-Pugnet, P.,Marza, E.,Fronzes, R.,Arfi, Y. (登録日: 2020-09-15, 公開日: 2021-04-07, 最終更新日: 2024-05-01)

主引用文献

Nottelet, P.,Bataille, L.,Gourgues, G.,Anger, R.,Lartigue, C.,Sirand-Pugnet, P.,Marza, E.,Fronzes, R.,Arfi, Y.
The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction.
Sci Adv, 7:-, 2021

PubMed Abstract: Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V and V domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.

PubMed: 33674316
DOI: 10.1126/sciadv.abf2403

実験手法

ELECTRON MICROSCOPY (3.5 Å)