7A7V

rsGreen1-K206A partially in the green-off state

7A7V の概要

• エントリーDOI: 10.2210/pdb7a7v/pdb
• 関連するPDBエントリー: 4XOV 4XOW
• 分子名称: Green fluorescent protein, GLYCEROL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: reversible photoswitchable fluorescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30847.53

構造登録者

De Zitter, E.,Dedecker, P.,Van Meervelt, L. (登録日: 2020-08-30, 公開日: 2021-02-17, 最終更新日: 2024-11-06)

主引用文献

De Zitter, E.,Hugelier, S.,Duwe, S.,Vandenberg, W.,Tebo, A.G.,Van Meervelt, L.,Dedecker, P.
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*.
Angew.Chem.Int.Ed.Engl., 60:10073-10081, 2021

PubMed Abstract: Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure-function relationships. We examined this using fluorescent proteins as easy-to-modify model systems. Starting from a single scaffold, we have developed a range of 27 photochromic fluorescent proteins that cover a broad range of spectroscopic properties, including the determination of 43 crystal structures. Correlation and principal component analysis confirmed the complex relationship between structure and spectroscopy, but also allowed us to identify consistent trends and to relate these to the spatial organization. We find that changes in spectroscopic properties can come about through multiple underlying mechanisms, of which polarity, hydrogen bonding and presence of water molecules are key modulators. We anticipate that our findings and rich structure/spectroscopy dataset can open opportunities for the development and evaluation of new and existing protein engineering methods.

PubMed: 33543524
DOI: 10.1002/anie.202015201

実験手法

X-RAY DIFFRACTION (2 Å)