7A0C
X-ray structure of NikA from Escherichia coli in complex with Fe-6-Me2-BPMCN
Summary for 7A0C
| Entry DOI | 10.2210/pdb7a0c/pdb |
| Descriptor | Nickel-binding periplasmic protein, FE (III) ION, 2-[[(1~{S},2~{S})-2-[methyl-[(6-methylpyridin-2-yl)methyl]amino]cyclohexyl]-[(6-methylpyridin-2-yl)methyl]amino]ethanoic acid, ... (8 entities in total) |
| Functional Keywords | artificial metalloenzyme, cross-linked enzyme crystal, sulfoxidation, metal binding protein |
| Biological source | Escherichia coli (strain K12) |
| Total number of polymer chains | 2 |
| Total formula weight | 115143.43 |
| Authors | Cavazza, C.,Menage, S. (deposition date: 2020-08-07, release date: 2020-11-04, Last modification date: 2024-01-31) |
| Primary citation | Lopez, S.,Marchi-Delapierre, C.,Cavazza, C.,Menage, S. A Selective Sulfide Oxidation Catalyzed by Heterogeneous Artificial Metalloenzymes Iron@NikA. Chemistry, 26:16633-16638, 2020 Cited by PubMed Abstract: Performing a heterogeneous catalysis with proteins is still a challenge. Herein, we demonstrate the importance of cross-linked crystals for sulfoxide oxidation by an artificial enzyme. The biohybrid consists of the insertion of an iron complex into a NikA protein crystal. The heterogeneous catalysts displays a better efficiency-with higher reaction kinetics, a better stability and expand the substrate scope compared to its solution counterpart. Designing crystalline artificial enzymes represents a good alternative to soluble or supported enzymes for the future of synthetic biology. PubMed: 33079395DOI: 10.1002/chem.202003746 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
Download full validation report
