7A0C

X-ray structure of NikA from Escherichia coli in complex with Fe-6-Me2-BPMCN

7A0C の概要

• エントリーDOI: 10.2210/pdb7a0c/pdb
• 分子名称: Nickel-binding periplasmic protein, FE (III) ION, 2-[[(1~{S},2~{S})-2-[methyl-[(6-methylpyridin-2-yl)methyl]amino]cyclohexyl]-[(6-methylpyridin-2-yl)methyl]amino]ethanoic acid, ... (8 entities in total)
• 機能のキーワード: artificial metalloenzyme, cross-linked enzyme crystal, sulfoxidation, metal binding protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 115143.43

構造登録者

Cavazza, C.,Menage, S. (登録日: 2020-08-07, 公開日: 2020-11-04, 最終更新日: 2024-01-31)

主引用文献

Lopez, S.,Marchi-Delapierre, C.,Cavazza, C.,Menage, S.
A Selective Sulfide Oxidation Catalyzed by Heterogeneous Artificial Metalloenzymes Iron@NikA.
Chemistry, 26:16633-16638, 2020

PubMed Abstract: Performing a heterogeneous catalysis with proteins is still a challenge. Herein, we demonstrate the importance of cross-linked crystals for sulfoxide oxidation by an artificial enzyme. The biohybrid consists of the insertion of an iron complex into a NikA protein crystal. The heterogeneous catalysts displays a better efficiency-with higher reaction kinetics, a better stability and expand the substrate scope compared to its solution counterpart. Designing crystalline artificial enzymes represents a good alternative to soluble or supported enzymes for the future of synthetic biology.

PubMed: 33079395
DOI: 10.1002/chem.202003746

実験手法

X-RAY DIFFRACTION (1.9 Å)