6ZX7

Antiparallel basket-type G-quadruplex DNA structure formed in human Bcl-2 promoter

6ZX7 の概要

• エントリーDOI: 10.2210/pdb6zx7/pdb
• NMR情報: BMRB: 34543
• 分子名称: bcl2ex (1 entity in total)
• 機能のキーワード: bcl2, g-quadruplex, promoter, dna
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7961.10

構造登録者

Bielskute, S.,Plavec, J.,Podbevsek, P. (登録日: 2020-07-29, 公開日: 2021-03-10, 最終更新日: 2024-06-19)

主引用文献

Bielskute, S.,Plavec, J.,Podbevsek, P.
Oxidative lesions modulate G-quadruplex stability and structure in the human BCL2 promoter.
Nucleic Acids Res., 49:2346-2356, 2021

PubMed Abstract: Misregulation of BCL2 expression has been observed with many diseases and is associated with cellular exposure to reactive oxygen species. A region upstream of the P1 promoter in the human BCL2 gene plays a major role in regulating transcription. This G/C-rich region is highly polymorphic and capable of forming G-quadruplex structures. Herein we report that an oxidative event simulated with an 8-oxo-7,8-dihydroguanine (oxoG) substitution within a long G-tract results in a reduction of structural polymorphism. Surprisingly, oxoG within a 25-nt construct boosts thermal stability of the resulting G-quadruplex. This is achieved by distinct hydrogen bonding properties of oxoG, which facilitate formation of an antiparallel basket-type G-quadruplex with a three G-quartet core and a G·oxoG·C base triad. While oxoG has previously been considered detrimental for G-quadruplex formation, its stabilizing effect within a promoter described in this study suggests a potential novel regulatory role of oxidative stress in general and specifically in BCL2 gene transcription.

PubMed: 33638996
DOI: 10.1093/nar/gkab057

実験手法

SOLUTION NMR