6ZV0
TFIIS N-terminal domain (TND) from human LEDGF/p75
Summary for 6ZV0
| Entry DOI | 10.2210/pdb6zv0/pdb |
| NMR Information | BMRB: 34537 |
| Descriptor | PC4 and SFRS1-interacting protein (1 entity in total) |
| Functional Keywords | chromatin reader, transcription |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 10648.39 |
| Authors | Veverka, V. (deposition date: 2020-07-23, release date: 2021-12-01, Last modification date: 2024-06-19) |
| Primary citation | Cermakova, K.,Demeulemeester, J.,Lux, V.,Nedomova, M.,Goldman, S.R.,Smith, E.A.,Srb, P.,Hexnerova, R.,Fabry, M.,Madlikova, M.,Horejsi, M.,De Rijck, J.,Debyser, Z.,Adelman, K.,Hodges, H.C.,Veverka, V. A ubiquitous disordered protein interaction module orchestrates transcription elongation. Science, 374:1113-1121, 2021 Cited by PubMed Abstract: During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS N-terminal domains (TNDs) and natively unstructured TND-interacting motifs (TIMs). This module was conserved among the elongation machinery and linked complexes including transcription factor TFIIS, Mediator, super elongation complex, elongin, IWS1, SPT6, PP1-PNUTS phosphatase, H3K36me3 readers, and other factors. Using nuclear magnetic resonance, live-cell microscopy, and mass spectrometry, we revealed the structural basis for these interactions and found that TND-TIM sequences were necessary and sufficient to induce strong and specific colocalization in the crowded nuclear environment. Disruption of a single TIM in IWS1 induced robust changes in gene expression and RNAP2 elongation dynamics, which underscores the functional importance of TND-TIM surfaces for transcription elongation. PubMed: 34822292DOI: 10.1126/science.abe2913 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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