6ZUC

X-RAY CRYSTAL STRUCTURE OF THE CsPYL1-Lig1-HAB1 TERNARY COMPLEX

6ZUC の概要

• エントリーDOI: 10.2210/pdb6zuc/pdb
• 分子名称: CSPYL1, Protein phosphatase 2C 16, 1,4-dimethyl-2-oxidanylidene-~{N}-(phenylmethyl)quinoline-6-sulfonamide, ... (7 entities in total)
• 機能のキーワード: cspyl1, hab1, phosphatase aba receptor inhibitor complex, plant protein
• 由来する生物種: Citrus sinensis (Sweet orange); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60611.50

構造登録者

Albert, A.,Infantes, L.,Benavente, J.L. (登録日: 2020-07-22, 公開日: 2021-08-04, 最終更新日: 2024-02-21)

主引用文献

Lozano-Juste, J.,Infantes, L.,Garcia-Maquilon, I.,Ruiz-Partida, R.,Merilo, E.,Benavente, J.L.,Velazquez-Campoy, A.,Coego, A.,Bono, M.,Forment, J.,Pampin, B.,Destito, P.,Monteiro, A.,Rodriguez, R.,Cruces, J.,Rodriguez, P.L.,Albert, A.
Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought.
Sci Adv, 9:eade9948-eade9948, 2023

PubMed Abstract: Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1, which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes.

PubMed: 36897942
DOI: 10.1126/sciadv.ade9948

実験手法

X-RAY DIFFRACTION (2.37 Å)