6ZSX

M2 mutant (R111K:Y134F:T54V:R132Q:P39Y:R59Y) of human cellular retinoic acid binding protein II - 4 conjugate

Summary for 6ZSX

• Entry DOI: 10.2210/pdb6zsx/pdb
• Related: 6Z2U 6Z2Z 6ZSW
• Descriptor: Cellular retinoic acid-binding protein 2, methyl (~{Z})-2-methyl-3-phenyl-prop-2-enoate (3 entities in total)
• Functional Keywords: human cellular retinoic acid binding protein ii, hcrabpii, conjigate, chromophore, m2, transport protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 16168.48

Authors

Tassone, G.,Pozzi, C.,Mangani, S. (deposition date: 2020-07-16, release date: 2021-07-28, Last modification date: 2024-10-16)

Primary citation

Tassone, G.,Paolino, M.,Pozzi, C.,Reale, A.,Salvini, L.,Giorgi, G.,Orlandini, M.,Galvagni, F.,Mangani, S.,Yang, X.,Carlotti, B.,Ortica, F.,Latterini, L.,Olivucci, M.,Cappelli, A.
Xanthopsin-Like Systems via Site-Specific Click-Functionalization of a Retinoic Acid Binding Protein.
Chembiochem, 23:e202100449-e202100449, 2022

PubMed Abstract: The use of light-responsive proteins to control both living or synthetic cells, is at the core of the expanding fields of optogenetics and synthetic biology. It is thus apparent that a richer reaction toolbox for the preparation of such systems is of fundamental importance. Here, we provide a proof-of-principle demonstration that Morita-Baylis-Hillman adducts can be employed to perform a facile site-specific, irreversible and diastereoselective click-functionalization of a lysine residue buried into a lipophilic binding pocket and yielding an unnatural chromophore with an extended π-system. In doing so we effectively open the path to the in vitro preparation of a library of synthetic proteins structurally reminiscent of xanthopsin eubacterial photoreceptors. We argue that such a library, made of variable unnatural chromophores inserted in an easy-to-mutate and crystallize retinoic acid transporter, significantly expand the scope of the recently introduced rhodopsin mimics as both optogenetic and "lab-on-a-molecule" tools.

PubMed: 34647400
DOI: 10.1002/cbic.202100449

Experimental method

X-RAY DIFFRACTION (2.4 Å)