6ZQJ

Cryo-EM structure of trimeric prME spike of Spondweni virus

6ZQJ の概要

• エントリーDOI: 10.2210/pdb6zqj/pdb
• 関連するPDBエントリー: 6ZQI
• EMDBエントリー: 11364 11366
• 分子名称: Genome polyprotein, prM, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: flavivirus, virus
• 由来する生物種: Spondweni virus; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 279602.76

構造登録者

Renner, M.,Dejnirattisai, W.,Carrique, L.,Serna Martin, I.,Karia, D.,Ilca, S.L.,Ho, S.F.,Kotecha, A.,Keown, J.R.,Mongkolsapaya, J.,Screaton, G.R.,Grimes, J.M. (登録日: 2020-07-09, 公開日: 2021-01-20, 最終更新日: 2024-10-23)

主引用文献

Renner, M.,Dejnirattisai, W.,Carrique, L.,Martin, I.S.,Karia, D.,Ilca, S.L.,Ho, S.F.,Kotecha, A.,Keown, J.R.,Mongkolsapaya, J.,Screaton, G.R.,Grimes, J.M.
Flavivirus maturation leads to the formation of an occupied lipid pocket in the surface glycoproteins.
Nat Commun, 12:1238-1238, 2021

PubMed Abstract: Flaviviruses such as Dengue (DENV) or Zika virus (ZIKV) assemble into an immature form within the endoplasmatic reticulum (ER), and are then processed by furin protease in the trans-Golgi. To better grasp maturation, we carry out cryo-EM reconstructions of immature Spondweni virus (SPOV), a human flavivirus of the same serogroup as ZIKV. By employing asymmetric localised reconstruction we push the resolution to 3.8 Å, enabling us to refine an atomic model which includes the crucial furin protease recognition site and a conserved Histidine pH-sensor. For direct comparison, we also solve structures of the mature forms of SPONV and DENV to 2.6 Å and 3.1 Å, respectively. We identify an ordered lipid that is present in only the mature forms of ZIKV, SPOV, and DENV and can bind as a consequence of rearranging amphipathic stem-helices of E during maturation. We propose a structural role for the pocket and suggest it stabilizes mature E.

PubMed: 33623019
DOI: 10.1038/s41467-021-21505-9

実験手法

ELECTRON MICROSCOPY (4.2 Å)