6ZOW

SARS-CoV-2 spike in prefusion state

6ZOW の概要

• エントリーDOI: 10.2210/pdb6zow/pdb
• EMDBエントリー: 11328
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: spike, prefusion, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 588064.89

構造登録者

Martinez, M.,Marabini, R.,Carazo, J.M. (登録日: 2020-07-07, 公開日: 2020-07-29, 最終更新日: 2024-10-16)

主引用文献

Melero, R.,Sorzano, C.O.S.,Foster, B.,Vilas, J.L.,Martinez, M.,Marabini, R.,Ramirez-Aportela, E.,Sanchez-Garcia, R.,Herreros, D.,Del Cano, L.,Losana, P.,Fonseca-Reyna, Y.C.,Conesa, P.,Wrapp, D.,Chacon, P.,McLellan, J.S.,Tagare, H.D.,Carazo, J.M.
Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures.
Iucrj, 7:1059-1069, 2020

PubMed Abstract: Using a new consensus-based image-processing approach together with principal component analysis, the flexibility and conformational dynamics of the SARS-CoV-2 spike in the prefusion state have been analysed. These studies revealed concerted motions involving the receptor-binding domain (RBD), N-terminal domain, and subdomains 1 and 2 around the previously characterized 1-RBD-up state, which have been modeled as elastic deformations. It is shown that in this data set there are not well defined, stable spike conformations, but virtually a continuum of states. An ensemble map was obtained with minimum bias, from which the extremes of the change along the direction of maximal variance were modeled by flexible fitting. The results provide a warning of the potential image-processing classification instability of these complicated data sets, which has a direct impact on the interpretability of the results.

PubMed: 33063791
DOI: 10.1107/S2052252520012725

実験手法

ELECTRON MICROSCOPY (3 Å)