6ZNO

The pointed end complex of dynactin with the p150 projection docked

6ZNO の概要

• エントリーDOI: 10.2210/pdb6zno/pdb
• 関連するPDBエントリー: 6ZO4
• EMDBエントリー: 11313 11314 11315 11316 11317 11318 11319
• 分子名称: ARP1 actin related protein 1 homolog A, ADENOSINE-5'-DIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE, ... (12 entities in total)
• 機能のキーワード: dynactin, complex, scaffold, cytoskeleton, structural protein
• 由来する生物種: Sus scrofa (Pig); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 343359.53

構造登録者

Lau, C.K.,Lacey, S.E.,Carter, A.P. (登録日: 2020-07-06, 公開日: 2020-07-29, 最終更新日: 2024-05-01)

主引用文献

Lau, C.K.,O'Reilly, F.J.,Santhanam, B.,Lacey, S.E.,Rappsilber, J.,Carter, A.P.
Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.
Embo J., 40:e106164-e106164, 2021

PubMed Abstract: Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In order to do this, it forms a tripartite complex with dynein and a coiled-coil adaptor. Dynactin consists of an actin-related filament whose length is defined by its flexible shoulder domain. Despite previous cryo-EM structures, the molecular architecture of the shoulder and pointed end of the filament is still poorly understood due to the lack of high-resolution information in these regions. Here we combine multiple cryo-EM datasets and define precise masking strategies for particle signal subtraction and 3D classification. This overcomes domain flexibility and results in high-resolution maps into which we can build the shoulder and pointed end. The unique architecture of the shoulder securely houses the p150 subunit and positions the four identical p50 subunits in different conformations to bind dynactin's filament. The pointed end map allows us to build the first structure of p62 and reveals the molecular basis for cargo adaptor binding to different sites at the pointed end.

PubMed: 33734450
DOI: 10.15252/embj.2020106164

実験手法

ELECTRON MICROSCOPY (6.8 Å)