6ZNH

Structure of the Salmonella PrgI needle filament attached to the basal body

6ZNH の概要

• エントリーDOI: 10.2210/pdb6znh/pdb
• EMDBエントリー: 11311
• 分子名称: PrgI (1 entity in total)
• 機能のキーワード: type 3 secretion system, salmonella, helical reconstruction, needle filament., protein transport
• 由来する生物種: Salmonella typhimurium
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 203891.96

構造登録者

Lunelli, M.,Kotov, V.,Wald, J.,Kolbe, M.,Marlovits, T.C. (登録日: 2020-07-06, 公開日: 2021-07-07, 最終更新日: 2024-05-01)

主引用文献

Kotov, V.,Lunelli, M.,Wald, J.,Kolbe, M.,Marlovits, T.C.
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.
Biochem Biophys Rep, 27:101039-101039, 2021

PubMed Abstract: Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.

PubMed: 34258394
DOI: 10.1016/j.bbrep.2021.101039

実験手法

ELECTRON MICROSCOPY (3.6 Å)