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6ZMV

Structure of muramidase from Trichobolus zukalii

6ZMV の概要
エントリーDOI10.2210/pdb6zmv/pdb
分子名称muramidase, GLYCEROL, SULFATE ION, ... (4 entities in total)
機能のキーワードmuramidase, fungal, gh25, lysozyme, industrial application, peptidoglycan cleavage, hydrolase
由来する生物種Trichobolus zukalii
タンパク質・核酸の鎖数2
化学式量合計46012.64
構造登録者
主引用文献Moroz, O.V.,Blagova, E.,Taylor, E.,Turkenburg, J.P.,Skov, L.K.,Gippert, G.P.,Schnorr, K.M.,Ming, L.,Ye, L.,Klausen, M.,Cohn, M.T.,Schmidt, E.G.W.,Nymand-Grarup, S.,Davies, G.J.,Wilson, K.S.
Fungal GH25 muramidases: New family members with applications in animal nutrition and a crystal structure at 0.78 angstrom resolution.
Plos One, 16:e0248190-e0248190, 2021
Cited by
PubMed Abstract: Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both β-1,4-N-acetyl- and β-1,4-N,6-O-diacetylmuramidase activities, cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A. alcalophilum enzyme at atomic, 0.78 Å resolution, together with that of its homologue from Trichobolus zukalii at 1.4 Å, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut.
PubMed: 33711051
DOI: 10.1371/journal.pone.0248190
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.4 Å)
構造検証レポート
Validation report summary of 6zmv
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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