6ZM8

Structure of muramidase from Acremonium alcalophilum

6ZM8 の概要

• エントリーDOI: 10.2210/pdb6zm8/pdb
• 分子名称: muramidase (2 entities in total)
• 機能のキーワード: muramidase, fungal, gh25, lysozyme, industrial application, peptidoglycan cleavage, hydrolase
• 由来する生物種: Sodiomyces alcalophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23050.13

構造登録者

Moroz, O.V.,Blagova, E.,Taylor, E.,Turkenburg, J.P.,Skov, L.K.,Gippert, G.P.,Schnorr, K.M.,Ming, L.,Ye, L.,Klausen, M.,Cohn, M.T.,Schmidt, E.G.W.,Nymand-Grarup, S.,Davies, G.J.,Wilson, K.S. (登録日: 2020-07-01, 公開日: 2021-07-14, 最終更新日: 2024-10-23)

主引用文献

Moroz, O.V.,Blagova, E.,Taylor, E.,Turkenburg, J.P.,Skov, L.K.,Gippert, G.P.,Schnorr, K.M.,Ming, L.,Ye, L.,Klausen, M.,Cohn, M.T.,Schmidt, E.G.W.,Nymand-Grarup, S.,Davies, G.J.,Wilson, K.S.
Fungal GH25 muramidases: New family members with applications in animal nutrition and a crystal structure at 0.78 angstrom resolution.
Plos One, 16:e0248190-e0248190, 2021

PubMed Abstract: Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both β-1,4-N-acetyl- and β-1,4-N,6-O-diacetylmuramidase activities, cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A. alcalophilum enzyme at atomic, 0.78 Å resolution, together with that of its homologue from Trichobolus zukalii at 1.4 Å, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut.

PubMed: 33711051
DOI: 10.1371/journal.pone.0248190

実験手法

X-RAY DIFFRACTION (0.78 Å)