6ZKC

Complex I during turnover, closed

6ZKC の概要

• エントリーDOI: 10.2210/pdb6zkc/pdb
• EMDBエントリー: 11244
• 分子名称: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial, NADH-ubiquinone oxidoreductase chain 6, NADH-ubiquinone oxidoreductase chain 4L, ... (58 entities in total)
• 機能のキーワード: complex, respiration, nadh, proton pump, mitochondria, iron-sulphur cluster, oxidoreductase, membrane protein, electron transport
• 由来する生物種: Ovis aries (Sheep); 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 1080815.11

構造登録者

Kampjut, D.,Sazanov, L.A. (登録日: 2020-06-30, 公開日: 2020-10-07, 最終更新日: 2025-04-09)

主引用文献

Kampjut, D.,Sazanov, L.A.
The coupling mechanism of mammalian respiratory complex I.
Science, 370:-, 2020

PubMed Abstract: Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different conditions, including turnover, at resolutions up to 2.3 to 2.5 angstroms. Resolved water molecules allowed us to experimentally define the proton translocation pathways. Quinone binds at three positions along the quinone cavity, as does the inhibitor rotenone that also binds within subunit ND4. Dramatic conformational changes around the quinone cavity couple the redox reaction to proton translocation during open-to-closed state transitions of the enzyme. In the induced deactive state, the open conformation is arrested by the ND6 subunit. We propose a detailed molecular coupling mechanism of complex I, which is an unexpected combination of conformational changes and electrostatic interactions.

PubMed: 32972993
DOI: 10.1126/science.abc4209

実験手法

ELECTRON MICROSCOPY (3.1 Å)