6ZK5

Plant nucleoside hydrolase - ZmNRh3 enzyme in complex with forodesine

6ZK5 の概要

• エントリーDOI: 10.2210/pdb6zk5/pdb
• 分子名称: Nucleoside N-ribohydrolase 3, CALCIUM ION, 1,4-DIDEOXY-4-AZA-1-(S)-(9-DEAZAHYPOXANTHIN-9-YL)-D-RIBITOL, ... (6 entities in total)
• 機能のキーワード: plant enzyme, hydrolase
• 由来する生物種: Zea mays (Maize)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72468.60

構造登録者

Morera, S.,Vigouroux, A.,Kopecny, D. (登録日: 2020-06-29, 公開日: 2022-01-12, 最終更新日: 2024-01-31)

主引用文献

Luptakova, E.,Vigouroux, A.,Koncitikova, R.,Kopecna, M.,Zalabak, D.,Novak, O.,Salcedo Sarmiento, S.,Cavar Zeljkovic, S.,Kopecny, D.J.,von Schwartzenberg, K.,Strnad, M.,Spichal, L.,De Diego, N.,Kopecny, D.,Morera, S.
Plant nucleoside N-ribohydrolases: riboside binding and role in nitrogen storage mobilization.
Plant J., 2023

PubMed Abstract: Cells save their energy during nitrogen starvation by selective autophagy of ribosomes and degradation of RNA to ribonucleotides and nucleosides. Nucleosides are hydrolyzed by nucleoside N-ribohydrolases (nucleosidases, NRHs). Subclass I of NRHs preferentially hydrolyzes the purine ribosides while subclass II is more active towards uridine and xanthosine. Here, we performed a crystallographic and kinetic study to shed light on nucleoside preferences among plant NRHs followed by in vivo metabolomic and phenotyping analyses to reveal the consequences of enhanced nucleoside breakdown. We report the crystal structure of Zea mays NRH2b (subclass II) and NRH3 (subclass I) in complexes with the substrate analog forodesine. Purine and pyrimidine catabolism are inseparable because nucleobase binding in the active site of ZmNRH is mediated via a water network and is thus unspecific. Dexamethasone-inducible ZmNRH overexpressor lines of Arabidopsis thaliana, as well as double nrh knockout lines of moss Physcomitrium patents, reveal a fine control of adenosine in contrast to other ribosides. ZmNRH overexpressor lines display an accelerated early vegetative phase including faster root and rosette growth upon nitrogen starvation or osmotic stress. Moreover, the lines enter the bolting and flowering phase much earlier. We observe changes in the pathways related to nitrogen-containing compounds such as β-alanine and several polyamines, which allow plants to reprogram their metabolism to escape stress. Taken together, crop plant breeding targeting enhanced NRH-mediated nitrogen recycling could therefore be a strategy to enhance plant growth tolerance and productivity under adverse growth conditions.

PubMed: 38044809
DOI: 10.1111/tpj.16572

実験手法

X-RAY DIFFRACTION (1.9 Å)