6ZIL

Structure of the isolated REC domain of RcsB from Salmonella enterica serovar Typhimurium in the apo form

6ZIL の概要

• エントリーDOI: 10.2210/pdb6zil/pdb
• 関連するPDBエントリー: 6ZII
• 分子名称: Transcriptional regulatory protein RcsB (2 entities in total)
• 機能のキーワード: response regulator, phosphorylation, two-component systems, transcriptional factor, dna binding protein
• 由来する生物種: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15703.31

構造登録者

Huesa, J.,Marina, A.,Casino, P. (登録日: 2020-06-26, 公開日: 2021-02-17, 最終更新日: 2024-01-31)

主引用文献

Huesa, J.,Giner-Lamia, J.,Pucciarelli, M.G.,Paredes-Martinez, F.,Portillo, F.G.,Marina, A.,Casino, P.
Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon.
Nucleic Acids Res., 49:2357-2374, 2021

PubMed Abstract: RcsB is a transcriptional regulator that controls expression of numerous genes in enteric bacteria. RcsB accomplishes this role alone or in combination with auxiliary transcriptional factors independently or dependently of phosphorylation. To understand the mechanisms by which RcsB regulates such large number of genes, we performed structural studies as well as in vitro and in vivo functional studies with different RcsB variants. Our structural data reveal that RcsB binds promoters of target genes such as rprA and flhDC in a dimeric active conformation. In this state, the RcsB homodimer docks the DNA-binding domains into the major groove of the DNA, facilitating an initial weak read-out of the target sequence. Interestingly, comparative structural analyses also show that DNA binding may stabilize an active conformation in unphosphorylated RcsB. Furthermore, RNAseq performed in strains expressing wild-type or several RcsB variants provided new insights into the contribution of phosphorylation to gene regulation and assign a potential role of RcsB in controlling iron metabolism. Finally, we delimited the RcsB box for homodimeric active binding to DNA as the sequence TN(G/A)GAN4TC(T/C)NA. This RcsB box was found in promoter, intergenic and intragenic regions, facilitating both increased or decreased gene transcription.

PubMed: 33638994
DOI: 10.1093/nar/gkab060

実験手法

X-RAY DIFFRACTION (3.12 Å)