6ZID

Ultrafast Structural Response to Charge Redistribution Within a Photosynthetic Reaction Centre - 5 ps (b) structure

6ZID の概要

• エントリーDOI: 10.2210/pdb6zid/pdb
• 関連するPDBエントリー: 6ZHW
• 分子名称: Photosynthetic reaction center cytochrome c subunit, BACTERIOCHLOROPHYLL B, BACTERIOPHEOPHYTIN B, ... (15 entities in total)
• 機能のキーワード: electron transport photosynthesis membrane protein, electron transport
• 由来する生物種: Blastochloris viridis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 145658.03

構造登録者

Baath, P.,Dods, R.,Braenden, G.,Neutze, R. (登録日: 2020-06-25, 公開日: 2020-12-09, 最終更新日: 2024-11-20)

主引用文献

Dods, R.,Bath, P.,Morozov, D.,Gagner, V.A.,Arnlund, D.,Luk, H.L.,Kubel, J.,Maj, M.,Vallejos, A.,Wickstrand, C.,Bosman, R.,Beyerlein, K.R.,Nelson, G.,Liang, M.,Milathianaki, D.,Robinson, J.,Harimoorthy, R.,Berntsen, P.,Malmerberg, E.,Johansson, L.,Andersson, R.,Carbajo, S.,Claesson, E.,Conrad, C.E.,Dahl, P.,Hammarin, G.,Hunter, M.S.,Li, C.,Lisova, S.,Royant, A.,Safari, C.,Sharma, A.,Williams, G.J.,Yefanov, O.,Westenhoff, S.,Davidsson, J.,DePonte, D.P.,Boutet, S.,Barty, A.,Katona, G.,Groenhof, G.,Branden, G.,Neutze, R.
Ultrafast structural changes within a photosynthetic reaction centre.
Nature, 589:310-314, 2021

PubMed Abstract: Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography using an X-ray free-electron laser to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions.

PubMed: 33268896
DOI: 10.1038/s41586-020-3000-7

実験手法

X-RAY DIFFRACTION (2.8 Å)