6ZGL

Structure of DPS determined by movement-free cryoEM with zero dose extrapolation

6ZGL の概要

• エントリーDOI: 10.2210/pdb6zgl/pdb
• EMDBエントリー: 11210
• 分子名称: DNA protection during starvation protein (2 entities in total)
• 機能のキーワード: dna-binding protein, dna binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 224643.54

構造登録者

Naydenova, K.,Russo, C.J. (登録日: 2020-06-19, 公開日: 2020-10-21, 最終更新日: 2024-05-01)

主引用文献

Naydenova, K.,Jia, P.,Russo, C.J.
Cryo-EM with sub-1 angstrom specimen movement.
Science, 370:223-226, 2020

PubMed Abstract: Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that this movement is caused by buckling and subsequent deformation of the suspended ice, with a threshold that depends directly on the shape of the frozen water layer set by the support foil. We describe a specimen support design that eliminates buckling and reduces electron beam-induced particle movement to less than 1 angstrom. The design allows precise foil tracking during imaging with high-speed detectors, thereby lessening demands on cryostage precision and stability. It includes a maximal density of holes, which increases throughput in automated cryo-EM without degrading data quality. Movement-free imaging allows extrapolation to a three-dimensional map of the specimen at zero electron exposure, before the onset of radiation damage.

PubMed: 33033219
DOI: 10.1126/science.abb7927

実験手法

ELECTRON MICROSCOPY (1.9 Å)