6ZFF

Pyrococcus furiosus Rad50 coiled coils in rod configuration

6ZFF の概要

• エントリーDOI: 10.2210/pdb6zff/pdb
• 分子名称: DNA double-strand break repair Rad50 ATPase, ZINC ION (3 entities in total)
• 機能のキーワード: double strand break repair, dna damge response, scaffold, closed coiled coil arms, dna
• 由来する生物種: Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32995.38

構造登録者

Soh, Y.M.,Basquin, J.,Gruber, S. (登録日: 2020-06-17, 公開日: 2020-07-08, 最終更新日: 2024-05-15)

主引用文献

Soh, Y.M.,Basquin, J.,Gruber, S.
A rod conformation of the Pyrococcus furiosus Rad50 coiled coil.
Proteins, 89:251-255, 2021

PubMed Abstract: The Rad50-Mre11 nuclease complex plays a vital role in DNA repair in all domains of life. It recognizes and processes DNA double-strand breaks. Rad50 proteins fold into an extended structure with a 20 to 60 nm long coiled coil connecting a globular ABC ATPase domain with a zinc hook dimerization domain. A published structure of an archaeal Rad50 zinc hook shows coiled coils pointing away from each other. Here we present the crystal structure of an alternate conformation displaying co-aligned coiled coils. Archaeal Rad50 may thus switch between rod-shaped and ring-like conformations as recently proposed for a bacterial homolog.

PubMed: 32875643
DOI: 10.1002/prot.26005

実験手法

X-RAY DIFFRACTION (3 Å)