6ZD2

Structure of apo telomerase from Candida Tropicalis truncated from C-terminal domain

6ZD2 の概要

• エントリーDOI: 10.2210/pdb6zd2/pdb
• 分子名称: Telomerase reverse transcriptase, SODIUM ION (2 entities in total)
• 機能のキーワード: telomerase, catalytic core, rna binding, telomere, replication
• 由来する生物種: Candida tropicalis MYA-3404
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 68882.25

構造登録者

Zhai, L.,Rety, S.,Chen, W.F.,Auguin, D.,Xi, X.G. (登録日: 2020-06-13, 公開日: 2021-04-28, 最終更新日: 2024-05-15)

主引用文献

Zhai, L.T.,Rety, S.,Chen, W.F.,Song, Z.Y.,Auguin, D.,Sun, B.,Dou, S.X.,Xi, X.G.
Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi‡.
Nucleic Acids Res., 49:4768-4781, 2021

PubMed Abstract: Telomerase plays critical roles in cellular aging, in the emergence and/or development of cancer, and in the capacity for stem-cell renewal, consists of a catalytic telomerase reverse transcriptase (TERT) and a template-encoding RNA (TER). TERs from diverse organisms contain two conserved structural elements: the template-pseudoknot (T-PK) and a helical three-way junction (TWJ). Species-specific features of the structure and function of telomerase make obtaining a more in-depth understanding of the molecular mechanism of telomerase particularly important. Here, we report the first structural studies of N-terminally truncated TERTs from Candida albicans and Candida tropicalis in apo form and complexed with their respective TWJs in several conformations. We found that Candida TERT proteins perform only one round of telomere addition in the presence or absence of PK/TWJ and display standard reverse transcriptase activity. The C-terminal domain adopts at least two extreme conformations and undergoes conformational interconversion, which regulates the catalytic activity. Most importantly, we identified a conserved tertiary structural motif, called the U-motif, which interacts with the reverse transcriptase domain and is crucial for catalytic activity. Together these results shed new light on the structure and mechanics of fungal TERTs, which show common TERT characteristics, but also display species-specific features.

PubMed: 33856462
DOI: 10.1093/nar/gkab261

実験手法

X-RAY DIFFRACTION (2.84 Å)