6ZCF

Amyloid fibril morphology i (in vitro) from murine SAA1.1 protein

6ZCF の概要

• エントリーDOI: 10.2210/pdb6zcf/pdb
• EMDBエントリー: 11162
• 分子名称: Serum amyloid A-2 protein (1 entity in total)
• 機能のキーワード: systemic amyloidosis, misfolding disease, inflammation, prion, protein fibril
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 139471.55

構造登録者

Bansal, A.,Schmidt, M.,Faendrich, M. (登録日: 2020-06-11, 公開日: 2021-02-17, 最終更新日: 2024-05-01)

主引用文献

Bansal, A.,Schmidt, M.,Rennegarbe, M.,Haupt, C.,Liberta, F.,Stecher, S.,Puscalau-Girtu, I.,Biedermann, A.,Fandrich, M.
AA amyloid fibrils from diseased tissue are structurally different from in vitro formed SAA fibrils.
Nat Commun, 12:1013-1013, 2021

PubMed Abstract: Systemic AA amyloidosis is a world-wide occurring protein misfolding disease of humans and animals. It arises from the formation of amyloid fibrils from serum amyloid A (SAA) protein. Using cryo electron microscopy we here show that amyloid fibrils which were purified from AA amyloidotic mice are structurally different from fibrils formed from recombinant SAA protein in vitro. Ex vivo amyloid fibrils consist of fibril proteins that contain more residues within their ordered parts and possess a higher β-sheet content than in vitro fibril proteins. They are also more resistant to proteolysis than their in vitro formed counterparts. These data suggest that pathogenic amyloid fibrils may originate from proteolytic selection, allowing specific fibril morphologies to proliferate and to cause damage to the surrounding tissue.

PubMed: 33579941
DOI: 10.1038/s41467-021-21129-z

実験手法

ELECTRON MICROSCOPY (2.73 Å)