6ZC2

Crystal structure of RahU protein in complex with TRIS molecule

6ZC2 の概要

• エントリーDOI: 10.2210/pdb6zc2/pdb
• 分子名称: RahU protein, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: aegerolysin, pseudomonas aeruginosa, rahu protein, protein-membrane interaction, toxin
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15936.37

構造登録者

Podobnik, M.,Anderluh, G.,Lenarcic, T. (登録日: 2020-06-09, 公開日: 2021-04-07, 最終更新日: 2024-01-24)

主引用文献

Kocar, E.,Lenarcic, T.,Hodnik, V.,Panevska, A.,Huang, Y.,Bajc, G.,Kostanjsek, R.,Naren, A.P.,Macek, P.,Anderluh, G.,Sepcic, K.,Podobnik, M.,Butala, M.
Crystal structure of RahU, an aegerolysin protein from the human pathogen Pseudomonas aeruginosa, and its interaction with membrane ceramide phosphorylethanolamine.
Sci Rep, 11:6572-6572, 2021

PubMed Abstract: Aegerolysins are proteins produced by bacteria, fungi, plants and protozoa. The most studied fungal aegerolysins share a common property of interacting with membranes enriched with cholesterol in combination with either sphingomyelin or ceramide phosphorylethanolamine (CPE), major sphingolipids in the cell membranes of vertebrates and invertebrates, respectively. However, genome analyses show a particularly high frequency of aegerolysin genes in bacteria, including the pathogenic genera Pseudomonas and Vibrio; these are human pathogens of high clinical relevance and can thrive in a variety of other species. The knowledge on bacterial aegerolysin-lipid interactions is scarce. We show that Pseudomonas aeruginosa aegerolysin RahU interacts with CPE, but not with sphingomyelin-enriched artificial membranes, and that RahU interacts with the insect cell line producing CPE. We report crystal structures of RahU alone and in complex with tris(hydroxymethyl)aminomethane (Tris), which, like the phosphorylethanolamine head group of CPE, contains a primary amine. The RahU structures reveal that the two loops proximal to the amino terminus form a cavity that accommodates Tris, and that the flexibility of these two loops is important for this interaction. We show that Tris interferes with CPE-enriched membranes for binding to RahU, implying on the importance of the ligand cavity between the loops and its proximity in RahU membrane interaction. We further support this by studying the interaction of single amino acid substitution mutants of RahU with the CPE-enriched membranes. Our results thus represent a starting point for a better understanding of the role of P. aeruginosa RahU, and possibly other bacterial aegerolysins, in bacterial interactions with other organisms.

PubMed: 33753805
DOI: 10.1038/s41598-021-85956-2

実験手法

X-RAY DIFFRACTION (1.13 Å)