6Z71

Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state

6Z71 の概要

• エントリーDOI: 10.2210/pdb6z71/pdb
• 分子名称: Aq128 (1 entity in total)
• 機能のキーワード: mate class transporter, membrane protein
• 由来する生物種: Aquifex aeolicus (strain VF5)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111134.31

構造登録者

Zhao, J.,Safarian, S.,Thielmann, Y.,Xie, H.,Wang, J.,Michel, H. (登録日: 2020-05-29, 公開日: 2021-12-01, 最終更新日: 2024-01-24)

主引用文献

Zhao, J.,Xie, H.,Mehdipour, A.R.,Safarian, S.,Ermler, U.,Munke, C.,Thielmann, Y.,Hummer, G.,Ebersberger, I.,Wang, J.,Michel, H.
The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Multidrug and toxic compound extrusion (MATE) transporters are widespread in all domains of life. Bacterial MATE transporters confer multidrug resistance by utilizing an electrochemical gradient of H or Na to export xenobiotics across the membrane. Despite the availability of X-ray structures of several MATE transporters, a detailed understanding of the transport mechanism has remained elusive. Here we report the crystal structure of a MATE transporter from at 2.0-Å resolution. In light of its phylogenetic placement outside of the diversity of hitherto-described MATE transporters and the lack of conserved acidic residues, this protein may represent a subfamily of prokaryotic MATE transporters, which was proven by phylogenetic analysis. Furthermore, the crystal structure and substrate docking results indicate that the substrate binding site is located in the N bundle. The importance of residues surrounding this binding site was demonstrated by structure-based site-directed mutagenesis. We suggest that Aq_128 is functionally similar but structurally diverse from DinF subfamily transporters. Our results provide structural insights into the MATE transporter, which further advances our global understanding of this important transporter family.

PubMed: 34753818
DOI: 10.1073/pnas.2107335118

実験手法

X-RAY DIFFRACTION (3.5 Å)