6Z38

TodX deltaS2S3 mutant monoaromatic hydrocarbon channel

6Z38 の概要

• エントリーDOI: 10.2210/pdb6z38/pdb
• 関連するPDBエントリー: 6Z34 6Z37
• 分子名称: TodX (1 entity in total)
• 機能のキーワード: fadl monoaromatic hydrocarbons biodegradation toluene outer membrane transport diffusion todx, membrane protein
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46264.48

構造登録者

van den Berg, B. (登録日: 2020-05-19, 公開日: 2020-11-04, 最終更新日: 2024-01-24)

主引用文献

Somboon, K.,Doble, A.,Bulmer, D.,Basle, A.,Khalid, S.,van den Berg, B.
Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion.
Nat Commun, 11:6331-6331, 2020

PubMed Abstract: In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology.

PubMed: 33303757
DOI: 10.1038/s41467-020-20126-y

実験手法

X-RAY DIFFRACTION (2.9 Å)