6Z37
TodX monoaromatic hydrocarbon channel deltaS2 mutant
Summary for 6Z37
| Entry DOI | 10.2210/pdb6z37/pdb |
| Related | 6Z34 |
| Descriptor | TodX (1 entity in total) |
| Functional Keywords | fadl monoaromatic hydrocarbons biodegradation toluene outer membrane transport diffusion todx, membrane protein |
| Biological source | Pseudomonas putida |
| Total number of polymer chains | 1 |
| Total formula weight | 46295.49 |
| Authors | van den Berg, B. (deposition date: 2020-05-19, release date: 2020-11-04, Last modification date: 2024-01-24) |
| Primary citation | Somboon, K.,Doble, A.,Bulmer, D.,Basle, A.,Khalid, S.,van den Berg, B. Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion. Nat Commun, 11:6331-6331, 2020 Cited by PubMed Abstract: In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology. PubMed: 33303757DOI: 10.1038/s41467-020-20126-y PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.05 Å) |
Structure validation
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