6Z0S

Allostery through DNA drives phenotype switching

6Z0S の概要

• エントリーDOI: 10.2210/pdb6z0s/pdb
• EMDBエントリー: 11022 12260
• 分子名称: comG promoter DNA - strand A, comG promoter DNA - strand B (2 entities in total)
• 機能のキーワード: transcription-factor, dna-binding, a-tract, allostery, dna binding protein
• 由来する生物種: Bacillus subtilis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57358.86

構造登録者

Rosenblum, G.,Elad, N.,Rozenberg, H.,Wiggers, F.,Jungwirth, J.,Hofmann, H. (登録日: 2020-05-11, 公開日: 2021-04-07, 最終更新日: 2024-05-22)

主引用文献

Rosenblum, G.,Elad, N.,Rozenberg, H.,Wiggers, F.,Jungwirth, J.,Hofmann, H.
Allostery through DNA drives phenotype switching.
Nat Commun, 12:2967-2967, 2021

PubMed Abstract: Allostery is a pervasive principle to regulate protein function. Growing evidence suggests that also DNA is capable of transmitting allosteric signals. Yet, whether and how DNA-mediated allostery plays a regulatory role in gene expression remained unclear. Here, we show that DNA indeed transmits allosteric signals over long distances to boost the binding cooperativity of transcription factors. Phenotype switching in Bacillus subtilis requires an all-or-none promoter binding of multiple ComK proteins. We use single-molecule FRET to demonstrate that ComK-binding at one promoter site increases affinity at a distant site. Cryo-EM structures of the complex between ComK and its promoter demonstrate that this coupling is due to mechanical forces that alter DNA curvature. Modifications of the spacer between sites tune cooperativity and show how to control allostery, which allows a fine-tuning of the dynamic properties of genetic circuits.

PubMed: 34016970
DOI: 10.1038/s41467-021-23148-2

実験手法

ELECTRON MICROSCOPY (5.7 Å)