6YVU

Condensin complex from S.cerevisiae ATP-free apo non-engaged state

6YVU の概要

• エントリーDOI: 10.2210/pdb6yvu/pdb
• EMDBエントリー: 10951
• 分子名称: Structural maintenance of chromosomes protein 2,Structural maintenance of chromosomes protein 2,Smc2, Structural maintenance of chromosomes protein 4, Condensin complex subunit 2,Condensin complex subunit 2,Brn1, ... (4 entities in total)
• 機能のキーワード: essential for the functional organization of genomes, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 519065.55

構造登録者

Lee, B.-G.,Cawood, C.,Gutierrez-Escribano, P.,Nakane, T.,Merkel, F.,Hassler, M.,Aragon, L.,Haering, C.H.,Lowe, J. (登録日: 2020-04-28, 公開日: 2020-07-15, 最終更新日: 2025-07-02)

主引用文献

Lee, B.G.,Merkel, F.,Allegretti, M.,Hassler, M.,Cawood, C.,Lecomte, L.,O'Reilly, F.J.,Sinn, L.R.,Gutierrez-Escribano, P.,Kschonsak, M.,Bravo, S.,Nakane, T.,Rappsilber, J.,Aragon, L.,Beck, M.,Lowe, J.,Haering, C.H.
Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
Nat.Struct.Mol.Biol., 27:743-751, 2020

PubMed Abstract: Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large loop domains, although the underlying molecular mechanisms have remained unknown. We used cryo-EM to investigate the nucleotide-driven reaction cycle of condensin from the budding yeast Saccharomyces cerevisiae. Our structures of the five-subunit condensin holo complex at different functional stages suggest that ATP binding induces the transition of the SMC coiled coils from a folded-rod conformation into a more open architecture. ATP binding simultaneously triggers the exchange of the two HEAT-repeat subunits bound to the SMC ATPase head domains. We propose that these steps result in the interconversion of DNA-binding sites in the catalytic core of condensin, forming the basis of the DNA translocation and loop-extrusion activities.

PubMed: 32661420
DOI: 10.1038/s41594-020-0457-x

実験手法

ELECTRON MICROSCOPY (7.5 Å)