6YU8

RNA Methyltransferase of Sudan Ebola Virus

6YU8 の概要

• エントリーDOI: 10.2210/pdb6yu8/pdb
• 分子名称: Methyltransferase domain of the L protein, VHH antiboby, GLYCEROL, ... (8 entities in total)
• 機能のキーワード: rna methyltransferase, virus, ebola, l protein, transferase
• 由来する生物種: Sudan virus - Boniface, Sudan,1976; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50994.05

構造登録者

Ferron, F.,Valle, C.,Zamboni, V.,Canard, B.,Decroly, E. (登録日: 2020-04-25, 公開日: 2021-02-17, 最終更新日: 2024-11-06)

主引用文献

Valle, C.,Martin, B.,Ferron, F.,Roig-Zamboni, V.,Desmyter, A.,Debart, F.,Vasseur, J.J.,Canard, B.,Coutard, B.,Decroly, E.
First insights into the structural features of Ebola virus methyltransferase activities.
Nucleic Acids Res., 49:1737-1748, 2021

PubMed Abstract: The Ebola virus is a deadly human pathogen responsible for several outbreaks in Africa. Its genome encodes the 'large' L protein, an essential enzyme that has polymerase, capping and methyltransferase activities. The methyltransferase activity leads to RNA co-transcriptional modifications at the N7 position of the cap structure and at the 2'-O position of the first transcribed nucleotide. Unlike other Mononegavirales viruses, the Ebola virus methyltransferase also catalyses 2'-O-methylation of adenosines located within the RNA sequences. Herein, we report the crystal structure at 1.8 Å resolution of the Ebola virus methyltransferase domain bound to a fragment of a camelid single-chain antibody. We identified structural determinants and key amino acids specifically involved in the internal adenosine-2'-O-methylation from cap-related methylations. These results provide the first high resolution structure of an ebolavirus L protein domain, and the framework to investigate the effects of epitranscriptomic modifications and to design possible antiviral drugs against the Filoviridae family.

PubMed: 33503246
DOI: 10.1093/nar/gkaa1276

実験手法

X-RAY DIFFRACTION (1.841 Å)