6YSS

Structure of the P+9 ArfB-ribosome complex in the post-hydrolysis state

これはPDB形式変換不可エントリーです。

6YSS の概要

• エントリーDOI: 10.2210/pdb6yss/pdb
• EMDBエントリー: 10906
• 分子名称: 50S ribosomal protein L32, 50S ribosomal protein L2, 50S ribosomal protein L3, ... (61 entities in total)
• 機能のキーワード: translation, ribosome, rescue, release
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2229018.78

構造登録者

Chan, K.-H.,Petrychenko, V.,Mueller, C.,Maracci, C.,Holtkamp, W.,Wilson, D.N.,Fischer, N.,Rodnina, M.V. (登録日: 2020-04-23, 公開日: 2020-08-19, 最終更新日: 2025-03-19)

主引用文献

Chan, K.H.,Petrychenko, V.,Mueller, C.,Maracci, C.,Holtkamp, W.,Wilson, D.N.,Fischer, N.,Rodnina, M.V.
Mechanism of ribosome rescue by alternative ribosome-rescue factor B.
Nat Commun, 11:4106-4106, 2020

PubMed Abstract: Alternative ribosome-rescue factor B (ArfB) rescues ribosomes stalled on non-stop mRNAs by releasing the nascent polypeptide from the peptidyl-tRNA. By rapid kinetics we show that ArfB selects ribosomes stalled on short truncated mRNAs, rather than on longer mRNAs mimicking pausing on rare codon clusters. In combination with cryo-electron microscopy we dissect the multistep rescue pathway of ArfB, which first binds to ribosomes very rapidly regardless of the mRNA length. The selectivity for shorter mRNAs arises from the subsequent slow engagement step, as it requires longer mRNA to shift to enable ArfB binding. Engagement results in specific interactions of the ArfB C-terminal domain with the mRNA entry channel, which activates peptidyl-tRNA hydrolysis by the N-terminal domain. These data reveal how protein dynamics translate into specificity of substrate recognition and provide insights into the action of a putative rescue factor in mitochondria.

PubMed: 32796827
DOI: 10.1038/s41467-020-17853-7

実験手法

ELECTRON MICROSCOPY (2.6 Å)