6YSH

Lamin A 1-70 coil1A dimer stabilized by C-terminal capping

6YSH の概要

• エントリーDOI: 10.2210/pdb6ysh/pdb
• 関連するPDBエントリー: 6YF5
• 分子名称: Prelamin-A/C,Microtubule-associated protein RP/EB family member 1 (3 entities in total)
• 機能のキーワード: intermediate filaments lamin coiled-coil, nuclear protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19387.59

構造登録者

Stalmans, G.,Lilina, A.V.,Strelkov, S.V. (登録日: 2020-04-22, 公開日: 2020-09-02, 最終更新日: 2024-01-24)

主引用文献

Stalmans, G.,Lilina, A.V.,Vermeire, P.J.,Fiala, J.,Novak, P.,Strelkov, S.V.
Addressing the Molecular Mechanism of Longitudinal Lamin Assembly Using Chimeric Fusions.
Cells, 9:-, 2020

PubMed Abstract: The molecular architecture and assembly mechanism of intermediate filaments have been enigmatic for decades. Among those, lamin filaments are of particular interest due to their universal role in cell nucleus and numerous disease-related mutations. Filament assembly is driven by specific interactions of the elementary dimers, which consist of the central coiled-coil rod domain flanked by non-helical head and tail domains. We aimed to investigate the longitudinal 'head-to-tail' interaction of lamin dimers (the so-called A interaction), which is crucial for filament assembly. To this end, we prepared a series of recombinant fragments of human lamin A centred around the N- and C-termini of the rod. The fragments were stabilized by fusions to heterologous capping motifs which provide for a correct formation of parallel, in-register coiled-coil dimers. As a result, we established crystal structures of two N-terminal fragments one of which highlights the propensity of the coiled-coil to open up, and one C-terminal rod fragment. Additional studies highlighted the capacity of such N- and C-terminal fragments to form specific complexes in solution, which were further characterized using chemical cross-linking. These data yielded a molecular model of the A complex which features a 6.5 nm overlap of the rod ends.

PubMed: 32645958
DOI: 10.3390/cells9071633

実験手法

X-RAY DIFFRACTION (2.83 Å)