Summary for 6YRX
| Entry DOI | 10.2210/pdb6yrx/pdb |
| Descriptor | Fatty acid Photodecarboxylase, STEARIC ACID, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total) |
| Functional Keywords | low-dose, room temperature, biosynthetic protein |
| Biological source | Chlorella variabilis (Green alga) |
| Total number of polymer chains | 1 |
| Total formula weight | 62441.08 |
| Authors | Sorigue, D.,Gotthard, G.,Blangy, S.,Nurizzo, D.,Royant, A.,Beisson, F.,Arnoux, P. (deposition date: 2020-04-20, release date: 2021-04-21, Last modification date: 2024-01-24) |
| Primary citation | Sorigue, D.,Hadjidemetriou, K.,Blangy, S.,Gotthard, G.,Bonvalet, A.,Coquelle, N.,Samire, P.,Aleksandrov, A.,Antonucci, L.,Benachir, A.,Boutet, S.,Byrdin, M.,Cammarata, M.,Carbajo, S.,Cuine, S.,Doak, R.B.,Foucar, L.,Gorel, A.,Grunbein, M.,Hartmann, E.,Hienerwadel, R.,Hilpert, M.,Kloos, M.,Lane, T.J.,Legeret, B.,Legrand, P.,Li-Beisson, Y.,Moulin, S.L.Y.,Nurizzo, D.,Peltier, G.,Schiro, G.,Shoeman, R.L.,Sliwa, M.,Solinas, X.,Zhuang, B.,Barends, T.R.M.,Colletier, J.P.,Joffre, M.,Royant, A.,Berthomieu, C.,Weik, M.,Domratcheva, T.,Brettel, K.,Vos, M.H.,Schlichting, I.,Arnoux, P.,Muller, P.,Beisson, F. Mechanism and dynamics of fatty acid photodecarboxylase. Science, 372:-, 2021 Cited by PubMed Abstract: Fatty acid photodecarboxylase (FAP) is a photoenzyme with potential green chemistry applications. By combining static, time-resolved, and cryotrapping spectroscopy and crystallography as well as computation, we characterized FAP reaction intermediates on time scales from subpicoseconds to milliseconds. High-resolution crystal structures from synchrotron and free electron laser x-ray sources highlighted an unusual bent shape of the oxidized flavin chromophore. We demonstrate that decarboxylation occurs directly upon reduction of the excited flavin by the fatty acid substrate. Along with flavin reoxidation by the alkyl radical intermediate, a major fraction of the cleaved carbon dioxide unexpectedly transformed in 100 nanoseconds, most likely into bicarbonate. This reaction is orders of magnitude faster than in solution. Two strictly conserved residues, R451 and C432, are essential for substrate stabilization and functional charge transfer. PubMed: 33833098DOI: 10.1126/science.abd5687 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.87 Å) |
Structure validation
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