6YQA

Taka-amylase in complex with alpha-glucosyl epi-cyclophellitol aziridine inhibitor

This is a non-PDB format compatible entry.

Summary for 6YQA

• Entry DOI: 10.2210/pdb6yqa/pdb
• Descriptor: Alpha-amylase, 2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (7 entities in total)
• Functional Keywords: inhibitor, complex, amylase, hydrolase
• Biological source: Aspergillus oryzae
• Total number of polymer chains: 2
• Total formula weight: 111495.60

Authors

Armstrong, Z.,Chen, Y.,Artola, M.,Overkleeft, H.,Davies, G. (deposition date: 2020-04-16, release date: 2021-02-24, Last modification date: 2024-01-24)

Primary citation

Chen, Y.,Armstrong, Z.,Artola, M.,Florea, B.I.,Kuo, C.L.,de Boer, C.,Rasmussen, M.S.,Abou Hachem, M.,van der Marel, G.A.,Codee, J.D.C.,Aerts, J.M.F.G.,Davies, G.J.,Overkleeft, H.S.
Activity-Based Protein Profiling of Retaining alpha-Amylases in Complex Biological Samples.
J.Am.Chem.Soc., 143:2423-2432, 2021

PubMed Abstract: Amylases are key enzymes in the processing of starch in many kingdoms of life. They are important catalysts in industrial biotechnology where they are applied in, among others, food processing and the production of detergents. In man amylases are the first enzymes in the digestion of starch to glucose and arguably also the preferred target in therapeutic strategies aimed at the treatment of type 2 diabetes patients through down-tuning glucose assimilation. Efficient and sensitive assays that report selectively on retaining amylase activities irrespective of the nature and complexity of the biomaterial studied are of great value both in finding new and effective human amylase inhibitors and in the discovery of new microbial amylases with potentially advantageous features for biotechnological application. Activity-based protein profiling (ABPP) of retaining glycosidases is inherently suited for the development of such an assay format. We here report on the design and synthesis of 1,6--cyclophellitol-based pseudodisaccharides equipped with a suite of reporter entities and their use in ABPP of retaining amylases from human saliva, murine tissue as well as secretomes from fungi grown on starch. The activity and efficiency of the inhibitors and probes are substantiated by extensive biochemical analysis, and the selectivity for amylases over related retaining endoglycosidases is validated by structural studies.

PubMed: 33497208
DOI: 10.1021/jacs.0c13059

Experimental method

X-RAY DIFFRACTION (1.67 Å)