6YQ4

Crystal structure of Fusobacterium nucleatum tannase

6YQ4 の概要

• エントリーDOI: 10.2210/pdb6yq4/pdb
• 分子名称: Tannase, GLYCEROL, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: tannase, colorectal cancer, pathogen, gallotannins, hydrolase
• 由来する生物種: Fusobacterium nucleatum subsp. nucleatum ATCC 25586
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 166049.41

構造登録者

Mancheno, J.M.,Anguita, J.,Rodriguez, H. (登録日: 2020-04-16, 公開日: 2021-03-24, 最終更新日: 2024-01-24)

主引用文献

Mancheno, J.M.,Atondo, E.,Tomas-Cortazar, J.,Luis Lavin, J.,Plaza-Vinuesa, L.,Martin-Ruiz, I.,Barriales, D.,Palacios, A.,Daniel Navo, C.,Sampedro, L.,Pena-Cearra, A.,Angel Pascual-Itoiz, M.,Castelo, J.,Carreras-Gonzalez, A.,Castellana, D.,Pellon, A.,Delgado, S.,Ruas-Madiedo, P.,de Las Rivas, B.,Abecia, L.,Munoz, R.,Jimenez-Oses, G.,Anguita, J.,Rodriguez, H.
A structurally unique Fusobacterium nucleatum tannase provides detoxicant activity against gallotannins and pathogen resistance.
Microb Biotechnol, 15:648-667, 2022

PubMed Abstract: Colorectal cancer pathogenesis and progression is associated with the presence of Fusobacterium nucleatum and the reduction of acetylated derivatives of spermidine, as well as dietary components such as tannin-rich foods. We show that a new tannase orthologue of F. nucleatum (TanBF ) has significant structural differences with its Lactobacillus plantarum counterpart affecting the flap covering the active site and the accessibility of substrates. Crystallographic and molecular dynamics analysis revealed binding of polyamines to a small cavity that connects the active site with the bulk solvent which interact with catalytically indispensable residues. As a result, spermidine and its derivatives, particularly N -acetylated spermidine, inhibit the hydrolytic activity of TanBF and increase the toxicity of gallotannins to F. nucleatum. Our results support a model in which the balance between the detoxicant activity of TanBF and the presence of metabolic inhibitors can dictate either conducive or unfavourable conditions for the survival of F. nucleatum.

PubMed: 33336898
DOI: 10.1111/1751-7915.13732

実験手法

X-RAY DIFFRACTION (2.399 Å)