6YLI

Crystal structure of human bcl-xL bound to trichoplax adhaerens trBak BH3

6YLI の概要

• エントリーDOI: 10.2210/pdb6yli/pdb
• 関連するPDBエントリー: 6YLD
• 分子名称: Bcl-2-like protein 1, Bcl-2 homologous antagonist/killer, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: bcl-2, bak, trichoplax adhaerens, apoptosis
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47397.71

構造登録者

D Sa, J.,Banjara, S.,Kvansakul, M. (登録日: 2020-04-07, 公開日: 2021-03-17, 最終更新日: 2024-01-24)

主引用文献

Popgeorgiev, N.,Sa, J.D.,Jabbour, L.,Banjara, S.,Nguyen, T.T.M.,Akhavan-E-Sabet, A.,Gadet, R.,Ralchev, N.,Manon, S.,Hinds, M.G.,Osigus, H.J.,Schierwater, B.,Humbert, P.O.,Rimokh, R.,Gillet, G.,Kvansakul, M.
Ancient and conserved functional interplay between Bcl-2 family proteins in the mitochondrial pathway of apoptosis.
Sci Adv, 6:-, 2020

PubMed Abstract: In metazoans, Bcl-2 family proteins are major regulators of mitochondrially mediated apoptosis; however, their evolution remains poorly understood. Here, we describe the molecular characterization of the four members of the Bcl-2 family in the most primitive metazoan, All four trBcl-2 homologs are multimotif Bcl-2 group, with trBcl-2L1 and trBcl-2L2 being highly divergent antiapoptotic Bcl-2 members, whereas trBcl-2L3 and trBcl-2L4 are homologs of proapoptotic Bax and Bak, respectively. trBax expression permeabilizes the mitochondrial outer membrane, while trBak operates as a BH3-only sensitizer repressing antiapoptotic activities of trBcl-2L1 and trBcl-2L2. The crystal structure of a trBcl-2L2:trBak BH3 complex reveals that trBcl-2L2 uses the canonical Bcl-2 ligand binding groove to sequester trBak BH3, indicating that the structural basis for apoptosis control is conserved from to mammals. Finally, we demonstrate that both trBax and trBak BH3 peptides bind selectively to human Bcl-2 homologs to sensitize cancer cells to chemotherapy treatment.

PubMed: 32998881
DOI: 10.1126/sciadv.abc4149

実験手法

X-RAY DIFFRACTION (1.9 Å)