6YLH の概要
| エントリーDOI | 10.2210/pdb6ylh/pdb |
| EMDBエントリー | 10839 |
| 分子名称 | 25S rRNA, 60S ribosomal protein L6-A, 60S ribosomal protein L7-A, ... (61 entities in total) |
| 機能のキーワード | pre-60s, biogenesis, lsu, large subunit, ribosome assembly, ribosome |
| 由来する生物種 | Saccharomyces cerevisiae (Baker's yeast) 詳細 |
| タンパク質・核酸の鎖数 | 60 |
| 化学式量合計 | 3358972.28 |
| 構造登録者 | |
| 主引用文献 | Kater, L.,Mitterer, V.,Thoms, M.,Cheng, J.,Berninghausen, O.,Beckmann, R.,Hurt, E. Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis. Mol.Cell, 79:615-628.e5, 2020 Cited by PubMed Abstract: Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix1-Ipi3 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression. PubMed: 32668200DOI: 10.1016/j.molcel.2020.06.032 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.1 Å) |
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