6YAP

Crystal structure of ZmCKO4a in complex with inhibitor 1-(3-Chloro-5-trifluoromethoxy-phenyl)-3-[2-(2-hydroxy-ethyl)-phenyl]-urea

6YAP の概要

• エントリーDOI: 10.2210/pdb6yap/pdb
• 分子名称: Cytokinin dehydrogenase 4, 1,2-ETHANEDIOL, FLAVIN-ADENINE DINUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: flavoprotein, cytokinin oxidase/dehydrogenase, phenyl-urea inhibitor, oxidoreductase
• 由来する生物種: Zea mays (Maize)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59840.65

構造登録者

Kopecny, D.,Briozzo, P.,Morera, S. (登録日: 2020-03-12, 公開日: 2020-10-14, 最終更新日: 2024-11-13)

主引用文献

Nisler, J.,Kopecny, D.,Pekna, Z.,Koncitikova, R.,Koprna, R.,Murvanidze, N.,Werbrouck, S.P.O.,Havlicek, L.,De Diego, N.,Kopecna, M.,Wimmer, Z.,Briozzo, P.,Morera, S.,Zalabak, D.,Spichal, L.,Strnad, M.
Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture.
J.Exp.Bot., 72:355-370, 2021

PubMed Abstract: Increasing crop productivity is our major challenge if we are to meet global needs for food, fodder and fuel. Controlling the content of the plant hormone cytokinin is a method of improving plant productivity. Cytokinin oxidase/dehydrogenase (CKO/CKX) is a major target in this regard because it degrades cytokinins. Here, we describe the synthesis and biological activities of new CKX inhibitors derived mainly from diphenylurea. They were tested on four CKX isoforms from maize and Arabidopsis, where the best compounds showed IC50 values in the 10-8 M concentration range. The binding mode of the most efficient inhibitors was characterized from high-resolution crystal complexed structures. Although these compounds do not possess intrinsic cytokinin activity, we have demonstrated their tremendous potential for use in the plant tissue culture industry as well as in agriculture. We have identified a key substance, compound 19, which not only increases stress resistance and seed yield in Arabidopsis, but also improves the yield of wheat, barley and rapeseed grains under field conditions. Our findings reveal that modulation of cytokinin levels via CKX inhibition can positively affect plant growth, development and yield, and prove that CKX inhibitors can be an attractive target in plant biotechnology and agriculture.

PubMed: 32945834
DOI: 10.1093/jxb/eraa437

実験手法

X-RAY DIFFRACTION (1.9 Å)